Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 17;26(1):223. doi: 10.3892/mmr.2022.12739

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Lin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 6. — Protein levels of (A) Notch1, (B) NICD1, (C) hairy and enhancer of split 1, (D) PTEN, (E-1) p-AKT (Thr308), (E-2) AKT, (E-3) p-AKT (Ser473), (F-1) p-mTOR (Ser2448), (F-2) mTORC1, (G) S6K1 (p70S6Kα), (H) S6K2 (p70S6Kβ) and (I) IL-17A in skin samples of experimental mice. *P<0.05 and **P<0.01. NICD1, Notch intracellular domain 1; p, phosphorylated; S6K, S6 kinase. Since protein bands of p-AKT (Thr308), p-AKT (Ser473) and AKT are not from the same western blot membrane, they are evaluated with each corresponding loading control, then the protein levels of p-AKT (Thr308) and p-AKT (Ser473) are calculated by comparing with that of AKT. Similarly, protein bands of p-mTOR (Ser2448) and mTORC1 are evaluated with each corresponding loading control, then the protein level of p-mTOR (Ser2448) is calculated by comparing with that of mTORC1.