This figure demonstrates the cumulative and time-varying associations between PM2.5 exposure over pregnancy and change in mtDNA mutational load: (A) total, and (B) nonsynonymous mutational load using BDLIM assuming day-specific effects. The y-axis represents the change in mtDNA mutational load corresponding to a μg/m3 increase in PM2.5; the x-axis is gestational age in days. Solid lines for time-varying associations show the predicted change in mtDNA mutational load. Gray areas indicate 95% confidence intervals (CIs). A sensitive window is identified for the days where the estimated pointwise 95% CI (shaded area) does not include zero. The models were adjusted for maternal age, education, race/ethnicity, child sex, and ambient temperature.