Fig. 1.

P2RX4 expression in murine lung tissue, human BALF and peripheral blood mononuclear cells. Relative P2rx4 (murine)/P2RX4 (human) expression determined via quantitative PCR in a) BALF cells and b) peripheral blood mononuclear cells obtained from COPD patients as well as in c) murine lung tissue isolated after cigarette smoke (CS) exposure for three consecutive days compared to non-smoking (Air/NS) individuals. P2X4 mRNA expression was quantified relative to ß2 microglobulin (ß2m) levels. mice: Air n = 9, CS n = 17; human BALF: n = 15 in both groups; human blood mononuclear cells (MNCs): never smokers n = 22, COPD n = 26. Each symbol represents individual subject. Graphs include mean with standard deviation unpaired t-Test with Welch’s correction was applied for group comparison. P is depicted in the corresponding graph and was considered statistical significant when ≤ 0.5