Fig. 3.

P2rx4-deficiency is associated with reduced airway inflammation and remodelling in response to regular cigarette smoke exposure for 4 months. a Differential cell count, and b concentrations of proteases in the BALF (ELA2, MMP-9) of P2rx4-deficient mice (P2rx4^−/−) after exposure with the smoke of 5 cigarettes per day, five days a week for four months compared to wild type animals (P2rx4^+/+). c Exemplary hematoxylin & eosin stained paraffin lung section (40 × magnification, 100 µm scale bar) used to quantify lung parenchyma destruction via calculating the linear mean intercept (Lm) and mean internal surface area (ISA). Mean Lm and ISA ± SDs are depicted under the corresponding histology pictures. BALF data is presented as individual symbols (n = 5) and mean + SD. Two-way ANOVA corrected for multiple comparison using Tukey test was applied for group comparison. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001