Fig. 4.

Blocking P2X4-signaling in THP-1 derived macrophages alleviates pro-inflammatory cytokine release in response to cigarette smoke extract stimulation. Concentrations of IL-1ß, TNFα, IL-6 and MCP-1 in the supernatant of THP-1 derived non-polarized macrophages treated with 10 µM of the specific P2X4-antagonist 5-BDBD 30 min prior to stimulation with 5% cigarette smoke extract compared to non-treated cells. Data is presented as individual symbols for 6 wells from three different experiments and mean + SD. Welch’s ANOVA corrected for multiple comparison using Dunett’s T3 was used for group comparison. *P < 0.05, **P < 0.01