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Effects of long-term cryopreservation on actived T cells and the T cell subtyes expressing CCR7 and CD45RA(n = 20). AThe proportion of CD38+HLA-DR+ T cells,Th and Tc. B The proportion of Th Naïve(CD45RA+CCR7+)Tcm(CD45−CCR7+)Tem(CCR7−CD45RA−)effector T(CD45RA+CCR−). C The proportion of Tc Naïve(CD45RA+CCR7+)Tcm(CD45−CCR7+)Tem(CCR7−CD45RA−)effector T(CD45RA+CCR−) in fresh isolated and cryopreserved (1, 3 and 6 mo, respectively) PBMCs. *P < 0.05, **P < 0.01,***P < 0.001
