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HPLC chromatogram of derivatized CoM (retention time, 2.9 min) from the hydrocarbon-contaminated aquifer. The buffer system used for both HPLC and LC-MS was ammonium acetate-acetonitrile (70:30). (Inset) LC-MS profile of the isoindole derivative of standard CoM (left panel) and the corresponding profile of the environmental sample (right panel). Both spectra show that the ionic molecular weight was 300, which is consistent with the structure of the fluorescent CoM derivative shown.
