TABLE 2.
CoM levels in various methanogens and CoM levels per cell
| Organism | Amt of CoM
|
Direct count procedure
|
MPN estimation procedure
|
|||
|---|---|---|---|---|---|---|
| nmol/mg of protein | nmol/ml | No. of cells/ml | fmol of CoM/cell | No. of cells/ml | fmol of CoM/cell | |
| Methanolobus tindarius | 11.49 | 0.56 | ||||
| Methanospirillum hungatei | 19.41 | 0.52 | 7.40 × 105 | 0.70 | 1.1 × 106 | 0.47 |
| Methanosarcina barkeri (grown on N2-CO2-methanol) | 41.11 | 1.97 | 4.60 × 106 | 0.43 | ||
| Methanosarcina barkeri (grown on N2-CO2-acetate) | 6.13 | 0.70 | 1.80 × 106 | 0.39 | ||
| Methanosarcina barkeri (grown on H2-CO2) | 18.21 | 1.78 | ||||
| Methanosaeta concilli | 6.59 | 0.38 | ||||
| Methanococcus voltae | 23.75 | 1.45 | 6.20 × 106 | 0.23 | ||
| Methanococcus thermolithotrophicus | 41.09 | 2.59 | 5.15 × 106 | 0.50 | 7.5 × 106 | 0.34 |
| Methanobacterium thermoautotrophicum Marburg | 7.48 | 0.40 | 2.85 × 106 | 0.14 | 1.1 × 107 | 0.36 |