FIGURE 5.

Effect of temperature pretreatment of fibril seeds on quiescent incubation curve. The human and mouse fibril seeds were pre-incubated under different temperature conditions (–20°C, 4°C, 37°C, and 90°C) for 2 h, then incubated with homologous monomers under 37°C. (A) The effect of temperature pretreatment of human seeds on the quiescent incubation curve, left. Corresponding ΔThT fluorescence value, right. (B) Effect of temperature pretreatment of mouse seeds on quiescent incubation curves, left. Corresponding ΔThT fluorescence value, right. Human and mouse α-synuclein fibril seeds still have the self-recruitment ability even after treatment at 90°C. After being treated at –20°C, the self-recruitment ability decreased significantly. All data are expressed in mean ± SD. Compared with the 37°C group, ***P < 0.001.