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Western blot analysis of recombinant β-glucosidase produced by a yeast transformant. The transformant was incubated in YNBD medium at 30°C for 1 day. The protein in the culture medium (lanes 1 and 3) and cell lysate (lanes 2 and 4) was detected by performing a Western blot analysis with anti-β-glucosidase antiserum. Lanes M contained high-molecular-mass markers.
