FIG. 6.

Gene structure and Southern blot analysis of A. kawachii IFO4308 (wild-type) bglA (A), a bglA disruptant (B), and a nonhomologous recombinant (C). Isolated genomic DNA was digested by PstI, and then a Southern blot analysis was performed by using a 368-bp PstI-SacI bglA fragment as the probe. Lane M contained a DNA molecular marker (λHindIII digest and φ174 HaeIII digest). The open box indicates the bglA ORF. The stippled boxes indicate 5′- and 3′-end-flanking regions. The hatched boxes indicate the sC gene of A. nidulans. The solid boxes indicate the regions where the probe bound.