FIG 3.
The N93/95A mutant exhibited impaired IFN antagonism compared with icPC22A. (A to C) Sensitivities of the recombinant PEDV N93/95A and icPC22A strains to IFN-β (A), IFN-λ1 (B), and IFN-λ3 (C) pretreatment of Vero cells. Cells were pretreated with different concentrations of IFNs and inoculated with the individual viruses at an MOI of 0.01 for 24 h. Supernatant and cells were collected and titrated for infectious virus titers in TCID50. (D to F) Relative cellular mRNA levels of swine IFN-β, IFN-λ1, and IFN-λ3. IPEC-DQ cells were inoculated with recombinant PEDVs (MOI of 3) or mock inoculated. Total cellular RNA was extracted at 9 hpi and quantified by reverse transcription followed by real-time PCR. The values are fold changes compared with mock treatment using the 2−ΔΔCT method. Data from three replicates are shown as mean ± SD.