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. 2022 May 11;96(11):e00398-22. doi: 10.1128/jvi.00398-22

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Copyright © 2022 Linville et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — B12 maintains VRK1 on chromatin during mitosis. (A) Immunofluorescence of CV1 cells transduced with empty-vector (CTRL)-, meGFP-, meGFP-VRK1-, and HA-B12-expressing lentiviruses. DAPI (nuclei), HA (B12), and GFP (alone or tagged to VRK1) were detected. (B) Immunofluorescence of mitotic CV1 cells transduced with empty vector (CTRL)-, meGFP-VRK1-, and HA-B12-expressing lentiviruses. Representative images from independent cell generations are shown. DAPI (DNA), H3Ser10(P), and GFP (VRK1) were detected. Arrow bars present in some panels to distinguish mitotic cells. Scale bar, 20 μm.