TABLE 2.
Antibody sources and dilutions
| Antibody | Source | Clone | Dilution | Catalog no. |
|---|---|---|---|---|
| Primary antibodies | ||||
| Rabbit polyclonal anti-BAF | Atlas Antibodies | BANF1 | 1:1,000 | HPA039242 |
| Mouse monoclonal anti-BAF | Santa Cruz Biotechnology | A-11 | 1:500 | sc-166324 |
| Rabbit polyclonal anti-BAF rabbit (total) | Laboratory of Matthew Wiebe | NAa | 1:3,000 | NA |
| Rabbit polyclonal anti-BAF rabbit (phosphorylated) | Laboratory of Matthew Wiebe | NA | 1:1,000 | NA |
| Rabbit polyclonal anti-D8 | Laboratory of Geoffrey Smith | NA | 1:1,000 | NA |
| Rabbit polyclonal anti-F17 | Laboratory of Paula Traktman | NA | 1:5,000 | NA |
| Mouse monoclonal anti-FLAG | Sigma Aldrich | M2 | 1:5,000 | F3165 |
| Rabbit polyclonal anti-FLAG | Sigma Aldrich | 1:5,000 | F7425 | |
| Rabbit polyclonal anti-GAPDH | Santa Cruz Biotechnology | FL-335 | 1:500 | sc-25778 |
| Mouse monoclonal anti-GAPDH | Sigma Aldrich | 71.1 | 1:1,000 | G8795 |
| Rabbit polyclonal anti-H2B | Santa Cruz Biotechnology | FL-126 | 1:500 | sc-10818 |
| Rabbit polyclonal anti-H3 | Cell Signaling | D1H2 | 1:1,000 | 4499S |
| Rabbit polyclonal anti-H3 | Cell Signaling | 1:1,000 | 9715 | |
| Rabbit polyclonal anti-H3Ser10(P) | Cell Signaling | 1:400 | 3377S | |
| Mouse monoclonal anti-HA | BioLegend | 16B12 | 1:1,000 | 901501 |
| Rabbit polyclonal anti-HA | Cell Signaling | C29F4 | 1:1,000 | 3724S |
| Rabbit polyclonal anti-HA | Sigma Aldrich | 1:1,000 | H6908 | |
| Rabbit polyclonal anti-I3 | Laboratory of Paula Traktman | 1:7,500 | NA | |
| Mouse monoclonal anti-IFIT2 | Santa Cruz Biotechnology | F-12 | 1:500 | sc-390724 |
| Mouse monoclonal anti-IFIT3 | Santa Cruz Biotechnology | B-7 | 1:500 | sc-393512 |
| Rabbit polyclonal anti-Ku70 | Cell Signaling | D10A7 | 1:1,000 | 4588S |
| Mouse monoclonal anti-Lamin A/C | Cell Signaling | 4C11 | 1:1,000 | 4777S |
| Mouse monoclonal anti-Lamin A/C | Abcam | 131C3 | 1:1,000 | ab8984 |
| Rabbit polyclonal anti-p53 | Cell Signaling | Z5F | 1:1,000 | 2527S |
| Mouse monoclonal anti-β-tubulin I | Sigma Aldrich | SAP.4G5 | 1:10,000 | T7816 |
| Rat monoclonal anti-α-tubulin | Serotec | YL1/2 | 1:10,000 | MCA77G |
| Mouse monoclonal anti-VRK1 | Abcam | 5D1 | 1:1,000 | ab171933 |
| Secondary antibodies | ||||
| Goat anti-mouse IgG HRP conjugate | BioRad | 1:20,000 | 170-6516 | |
| Goat anti-rabbit IgG HRP conjugate | BioRad | 1:20,000 | 170-6515 | |
| Alexa Fluor 647 goat anti-rabbit IgG | Life Technologies | 1:400 | A21245 | |
| Alexa Fluor 488 goat anti-mouse IgG | Life Technologies | 1:400 | A11029 | |
| IRDye 680RD-conjugated goat anti-rabbit IgG | LI-COR | 1:10,000 | 926-68071 | |
| IRDye 680LT-conjugated goat anti-mouse IgG | LI-COR | 1:10,000 | 926-68020 | |
| IRDye 800CW-conjugated goat anti-rabbit IgG | LI-COR | 1:10,000 | 926-32211 | |
| IRDye 800CW-conjugated goat anti-mouse IgG | LI-COR | 1:10,000 | 926-32210 | |
| IRDye 680LT-conjugated goat anti-rat IgG | LI-COR | 1:10,000 | 926-68029 |
a
NA, not applicable.