Figure 1. AR deletion in SMCs of the normal adult prostate.

(A) Timeline of experiments for the wt and str^AR− mice.

(B and C) IF staining showing YFP-marked cells present only in the stroma (B) and positive for SMA (C).

(D and E) IF staining showing loss of AR expression in YFP⁺ cells from wt (D) to str^AR− (E). Zoom-in views of the boxed areas are shown on the right.

(F) Quantitation of the proportions of AR⁺ SMCs in different prostate lobes before and after AR deletion through time.

(G) Quantitation of prostate lobe weights in wt and str^AR− mice 9 months after induction.

(H) Representative IF staining of BrdU (left) and quantitation of the proportions of BrdU⁺ cells in AR⁺ and AR⁻ SMCs (right). Arrowhead points to an AR⁻BrdU⁺ SMC and arrow to an AR⁺BrdU⁺ SMC.

(I) H&E staining showing prostate morphology of str^AR− mice 9 months after induction. Arrows point to regions of hyperplasia. *p < 0.05 and **p < 0.001 by Student’s t test. Scale bars, 20 μm.