KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-AR (1:500) | Sigma-Aldrich | Cat#A9853; RRID: AB_262132 |
| rat anti-BrdU (1:500) | Bio-Rad | Cat# MCA2060; RRID: AB_323427 |
| rabbit anti-CK5 (1:1000) | Covance | Cat# PRB-160P-100; RRID: AB_291581 |
| mouse anti-CK18 (1:100) | Abcam | Cat# ab668; RRID: AB_305647 |
| mouse anti-SMA (1:500) | Sigma-Aldrich | Cat# A2547; RRID: AB_476701 |
| chick anti-YFP (1:2000) | Abcam | Cat# ab13970; RRID: AB_300798 |
| rabbit anti-Pten (1:100) | Cell Signaling Technology | Cat# 9559; RRID: AB_390810 |
| rabbit anti-pAkt(Ser473) (1:50) | Cell Signaling Technology | Cat# 4060; RRID: AB_2315049 |
| rabbit anti-pAkt(Thr308) (1:100) | Cell Signaling Technology | Cat# 13038; RRID: AB_2629447 |
| rabbit anti-pRPS6(Ser235/236) (1:400) | Cell Signaling Technology | Cat# 2211; RRID: AB_331679 |
| rabbit anti-p4E-BP1(Thr37/46) (1:1600) | Cell Signaling Technology | Cat# 2855; RRID: AB_560835 |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat#T5648-5G |
| BrdU | Sigma-Aldrich | Cat#5002-5G |
| Testosterone | Sigma-Aldrich | Cat#T1500-1G |
| Estradiol-17β | Sigma-Aldrich | Cat#E8875-1G |
| Critical commercial assays | ||
| DetectX Serum 17β-Estradiol Enzyme Immunoassay Kit | Arbor Assays | Cat#KB30-H1 |
| Vectastain Elite ABC HRP Kit | Vector Labs | Cat#PK-6101 |
| Vector NovaRED Substrate Kit | Vector Labs | Cat#SK-4800 |
| Chromium Single Cell 3′ Reagent Kits (v3.1 Chemistry) | 10x Genomics | Cat#1000121, 1000127 |
| Deposited data | ||
| scRNA-seq raw data | This paper | GEO: GSE186114 |
| Experimental models: Organisms/strains | ||
| Tg(Myh11-icre/ERT2)1Soff | Jackson Laboratory (Wirth et al., 2008) | Strain #019079 |
| B6.Cg-Gt(ROSA)26Sortm3(CAG–EYFP)Hze/J | Jackson Laboratory (Madisen et al., 2010) | Strain #007903 |
| ARflox/Y | De Gendt et al. (2004) | Kindly provided by Dr. Michael Shen |
| Hi-Myc | Ellwood-Yen et al. (2003) | Kindly provided by Dr. Michael Shen |
| Oligonucleotides | ||
| Genotyping primer for Myh11-CreERT2 forward: TGACCCCATCTCTTCACTCC | This paper | N/A |
| Genotyping primer for Myh11-CreERT2 reverse: AGTCCCTCACATCCTCAGGTT | This paper | N/A |
| Genotyping primer for ARflox forward: GTTGATACCTTAACCTCTGC | This paper | N/A |
| Genotyping primer for ARflox reverse: CTTCAGCGGCTCTTTTGAAG | This paper | N/A |
| Genotyping primer for Hi-Myc forward: AAACATGATGACTACCAAGCTTGGC | This paper | N/A |
| Genotyping primer for Hi-Myc reverse: ATGATAGCATCTTGTTCTTAGTCTTTTTCTTAATAGGG | This paper | N/A |
| Genotyping primer for R26R-CAG-EYFP wt forward: AAGGGAGCTGCAGTGGAGTA | This paper | N/A |
| Genotyping primer for R26R-CAG-EYFP wt reverse: CCGAAAATCTGTGGGAAGTC | This paper | N/A |
| Genotyping primer for R26R-CAG-EYFP mutated forward: ACATGGTCCTGCTGGAGTTC | This paper | N/A |
| Genotyping primer for R26R-CAG-EYFP mutated reverse: GGCATTAAAGCAGCGTATCC | This paper | N/A |
| Software and algorithms | ||
| GraphPad Prism 8.0 | GraphPad | RRID: SCR_002798 |
| 10x Genomics Cell Ranger pipeline v5.0.1 | 10x Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation |
| Scanpy v1.7.1 | Wolf et al. (2018) | RRID: SCR_018139 |
| Scrublet v0.2.1 | Wolock et al. (2019) | RRID: SCR_018098 |
| scVI-tools v0.7.0 | Gayoso et al. (2021) | https://scvi-tools.org |
| GSEA v4.1.0 | Subramanian et al. (2005) | RRID: SCR_003199 |