TABLE 1.
Oligonucleotide probe sequences used in slot blot and fluorescent hybridization experiments
| Probe | Sequence (5′ to 3′) | Target (E. coli positions) | Specificity | Reference |
|---|---|---|---|---|
| UNIV522 | GWATTACCGCGGCKGCTG | 522–540 | All bacteria | 25 |
| EUB338 | GCTGCCTCCCGTAGGAGT | 338–355 | Eubacteria | 4 |
| ARCH915 | GTGCTCCCCCGCCAATTCCT | 915–935 | Archaebacteria | 34 |
| Nonsense | ACTCCTACGGGAGGCAGC | 338–355 | Control | 4 |
| Eub-1 | GGGAACTCCACTCCCCTG | 664–681 | OTU Eub-1 | This study |
| Eub-2 | ACTGGTTGTGCGCCTCC | 927–942 | OTU Eub-2 | This study |
| Eub-3 | AATCTTTACTCTAAAACACAC | 197–218 | OTU Eub-3 | This study |
| Eub-4 | CCACCTCCCTCTACCATCC | 656–674 | OTU Eub-4 | This study |
| Eub-5 (SRB385) | CGGCGTCGCTGCGTCAGG | 385–402 | Desulfovibrio | 4 |
| Eub-6 | CCACTCCCCTCTGTCACC | 657–674 | OTU Eub-6 | This study |
| Arch-1 (MX825) | TCGCACCGTGGCCGACACCTAGC | 825–847 | Methanosaeta | 34 |
| Arch-2 | ATAGTCTATGGGGTATTATC | 171–190 | OTU Arch-2 | This study |