Table 4. Model Predictions and Experimental Values of CQAs, Cellular Activation, and Protein Expression for the Optimized LNP Formulations.
| optimal
1 |
optimal
2 |
optimal
3 |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| output | predicted | mRNA | saRNA | predicted | mRNA | saRNA | predicted | mRNA | saRNA |
| EE (%) | 81.42 | 76.73 | 77.97 | 80.27 | 82.00 | 87.91 | 92.03 | 86.10 | 92.29 |
| size (nm) | 104.83 | 82.62 | 87.72 | 92.83 | 85.56 | 85.51 | 100.00 | 81.70 | 81.45 |
| RNA integrity (% full transcript) | 48.74 | 73.87 | 54.80 | 42.27 | 73.10 | 47.65a | 49.43 | 84.23 | 52.30 |
| IL-6 release (pg/mL) | 780 | 872 | 771 | 1424 | 1324 | 1478 | 881 | 823 | 782 |
| protein expression (RLU) | 2.01 × 107 | 5.65 × 104 | 2.04 × 107 | 2.42 × 107 | 3.61 × 105 | 1.17 × 107 | 1.80 × 107 | 3.55 × 104 | 1.32 × 107 |
| overall desirability | 0.8228 | 0.9165 | 0.9203 | ||||||
a
One outlier replicate was removed from analysis due to highly apparent degradation by RNase.