Figure 3 |. MiN tau protein profile mirrors that of the adult human brain.

(A) Quantitation of relative tau peptides from multiple independent samples as indicated in the plot legend. Mean(thick line)±SEM. Pink and red dashed outline depicts1N, 1N/2N, and 4R-specific tau peptides, respectively. (B) Quantification of 3R:4R isoform ratio of all samples used in Figure 3A. Histogram colors are matched to sample colors shown in the plot legend of Figure 3A. Mean±SEM; One-Way ANOVA with multiple comparisons and post hoc Tukey’s test: Between any fetal vs adult sample for all three 4R peptides: ***p ≤ 0.001. (C) Percent of N isoforms (calculation in methods) using all samples as indicated in the plot legend in Figure 3A. Mean±SEM; One-Way ANOVA with multiple comparisons and post hoc Tukey’s test. All N isoforms between fetal vs healthy iPSC-Ns and between adult vs healthy miNs: ns p ¾ 0.999; between healthy iPSC-Ns vs healthy miNs: 0N: **p = 0.002, 1N: **p = 0.002, 2N: *p = 0.015. (D) Western blot of tau isoforms. (E) Left, western blot of tau isoforms over reprogramming timecourse. Right, quantification of 3R:4R ratio of 0N and 1N isoforms during the time course of reprogramming of two independent samples indicated in the plot legend. Mean±SEM; One-Way ANOVA with multiple comparisons and post hoc Tukey’s test; Between fetal vs any miN: **p = 0.002; between fetal and adult: **p = 0.004.