Figure 1.

Pep1 induces callose and lignin depositions in roots and shoots. (a) Pep1 induces callose deposition in the vasculature of shoot and root. (b) Pep1 induces lignin deposition in the vasculature of shoot and root. One-week-old plate-grown seedlings of WT or pmr4 mutants were treated with H₂O, 1 μM Pep1, 1 μM SCOOP12, or 1 μM flg22 for 24 hours, followed by callose (A) or lignin staining (B). At least ten seedlings for each treatment were detected with similar results. Bar = 200 μm. All experiments were repeated three times with similar results.