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. 2022 Feb 15;17(1):2034270. doi: 10.1080/15592324.2022.2034270

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Pep1-induced callose and lignin deposition relies on PEPR1/2, BAK1/SERK4, BIK1/PBL1 and AGB1. (a and b) Pep1 induces callose and lignin depositions through PEPR1/2, BAK1/SERK4, and BIK1/PBL1. (c and d) PEPR2 plays a major role in the Pep1-induced lignin disposition. (e and f) Pep1, Pep2, but not Pep3 induce lignin disposition in root tips.(g-h) Pep1 induces callose and lignin deposition through AGB1. One-week-old plate-grown seedlings of indicated genotypes were treated with H₂O or 1 μM Pep1 for 24 hours, followed by callose or lignin staining. Quantification data of callose and lignin in b, d, f, and h were indicated as means of intensity unit from each repeat. Significant differences were shown by different letters (Student’s t-test, n ≥ 8). The experiment was repeated three times with similar results. Bar = 200 μm.