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JA and ET signaling oppositely regulate Pep1-induced expression of genes involved in callose and lignin biosynthesis. One-week-old seedlings of indicated genotypes were treated with 1 μM Pep1, mRNA was isolated for RT-qPCR analysis of gene transcription levels 6 hours post treatment. Different letters indicate a significant difference with others (P < .01, Student’s t-test, n = 3). The experiments were repeated three times with similar results.
