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. 2022 May 30;19(1):751–763. doi: 10.1080/15476286.2022.2079889

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — DARS-AS1 binds to DARS 5ʹUTR to regulate DARS mRNA stability. A Subcellular fractionation assays identified the cellular location of DARS-AS1 in HeLa and C33A cells treated with hypoxia. B FISH assays detected the co-existence of DARS-AS1 and DARS in HeLa and C33A cells treated with hypoxia. C Luciferase activity of DARS promoter in DARS-AS1-silenced HeLa and C33A cells treated with hypoxia was analysed. D Combination between DARS 5ʹUTR and DARS-AS1 in hypoxia-treated cells was confirmed using RNA pull down assay. E Stability of DARS mRNA in DARS-AS1-silenced cells treated with hypoxia in the presence of actinomycin D was analysed. Experimental procedures were repeated independently for three times. *P < 0.05, **P < 0.01.