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Process optimization of suspensive AAV production. The pAAV plasmid carrying 3.9 kb of NLuc fused genes was used to transfect HEK 293F cells with duplication. (A) Effect of transfection viable cell density (VCD) on AAV production. (B) Effect of ratio of plasmid DNA (μg) and host cell number (106 cells). (C) Effect of gene size cloned in pAAV expression plasmid. Both 3.9 kb and 1.1 kb of insert genes were tested. **P ≤ 0.05.
