Table 1.

Summary of tested AAV production parameters.

Production mode	Host cells	Transfection reagent	Transfection VCD (x106 c/mL)	DNA:cell (μg:106)	Gene size (kb)	Container and scale	AAV volumetric productivity (gc/mL)
Adherent	293AAV	Calcium	0.4	3.3	3.9	Petri dish, 30 mL	3.87-7.86±0.54x109
		Lipofectamine	0.4	3.3	3.9	Petri dish, 30 mL	8.37±3.02x108
		Liposomes	0.4	3.3	3.9	Petri dish, 30 mL	5.29±1.02x108
	293A	Calcium	0.4	3.3	3.9	Petri dish, 30 mL	6.25±1.31x109
	293F	Calcium	0.4	3.3	3.9	Petri dish, 30 mL	3.01±0.25x109
Suspensive	293F	PEI	0.4	3.3	3.9	Shaker flask, 30 mL	5.38±0.65x108
	293F	TurboFect	0.4	3.3	3.9	Shaker flask, 30 mL	2.83±0.43x107
	293F	Liposomes	0.4	3.3	3.9	Shaker flask, 30 mL	5.78±0.56x109
	293F	Liposomes	0.2	1.6	3.9	Shaker flask, 30 mL	2.20±0.36x109
	293F	Liposomes	0.4	1.6	3.9	Shaker flask, 30 mL	5.55±1.01x109
	293F	Liposomes	0.5	1.6	3.9	Shaker flask, 30 mL	6.46±0.22x108
	293F	Liposomes	0.6	1.6	3.9	Shaker flask, 30 mL	2.98±0.18x108
	293F	Liposomes	0.4	1.6	3.9	Shaker flask, 30 mL	2.20±0.36x109
	293F	Liposomes	0.4	3.3	3.9	Shaker flask, 30 mL	1.33±0.03x109
	293F	Liposomes	0.4	6.6	3.9	Shaker flask, 30 mL	1.12±0.08x109
	293F	Liposomes	0.4	1.6	3.9	Shaker flask, 30 mL	7.64±0.99x109
	293F	Liposomes	0.4	1.6	1.1	Shaker flask, 30 mL	4.56±0.06x109
	293F	Liposomes	0.4	1.6	3.9	Shaker flask, 240 mL	5.04±0.63x109
	293F	Liposomes	0.4	1.6	3.9	Spinner flask, 450 mL	4.99±0.82x109

Note: All data are presented as mean ± standard error of the mean (SEM).