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. 2022 Mar 17;8(3):000788. doi: 10.1099/mgen.0.000788

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© 2022 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 3. — Diversity of integron integrases recovered by the intI-R / HS286 primer set. (a) Total non-redundant (100 % amino acid identity) integron integrases (IntIs) recovered. (b) IntI diversity was normalised for sequencing, based on averages (±1 S.E) of triplicate 50-megabase subsamples of raw sequence reads. Total (c) and normalised (d) diversity of IntI classes (using a 94 % amino acid clustering threshold) are shown. Average (±1 S.E) diversity for each analysis are shown on the right-hand side of each panel. Differences between Nanopore (ONT) and Illumina MiSeq technologies were not significant (NS) as determined by Wilcoxon rank sum tests.