Fig. 1.

Summary of sequencing methods and output data. (a) Comparison of SRA and LRA amplicons. SRA consists of a portion of the 16S rRNA gene spanning variable regions 3 and 4. LRA spans the entire 16S rRNA gene, the ITS region, and part of the 23S rRNA gene. (b) Summary of the four sequencing methods compared in this analysis, including the sequencing platforms, read lengths, data outputs and multiplexing capabilities. (c) Number of reads passing demultiplexing and filtering for SRA and the number of HiFi reads passing demultiplexing and classification for LRA by sample replicate. For LRA, the DNA extraction method used for each replicate is denoted in parentheses in the legend. (d) The number of raw read pairs and the number of read pairs passing filtering for SRM. (e) The number of LRM reads and the average read length per sample. Participant 6's treatment sample failed LRM library preparation and was excluded from analysis.