Fig. 2.

Sanger sequencing and analysis. (a) The Sanger sequencing workflow. cDNA, complementary DNA; PCR, polymerase chain reaction; BDT, BigDye Terminator; BDX, BigDye Xterminator; CE, capillary electrophoresis. (b) Violin plot showing the distribution of RT-PCR cycle threshold scores for 222 samples; the C _t score for each sample was based on the mean C _t of the three SARS-CoV-2 targets (S gene, N gene, Orf1ab). Quartiles are represented by different shades within each plot, mean and median C _ts are represented by yellow and pink points, respectively. The pink line shows the trend line.