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. 2022 Mar 16;8(3):000774. doi: 10.1099/mgen.0.000774

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© 2022 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 3. — Summary of the workflow for improving the coverage of WGS data using Sanger sequencing. Data from the sequencer are preprocessed to determine coverage. Sequences with gaps in regions of interest are sent for Sanger sequencing. The results from Sanger sequencing are inserted into the NGS whole genomes using SeqPatcher and the improved consensus of these is published to support genomic epidemiology.