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. 2022 May 25;13:926885. doi: 10.3389/fphys.2022.926885

FIGURE 6.

FIGURE 6

Localization of CA transcripts in the gill tissue of M. nipponense by in situ hybridization. Sense probes were used as negative controls, which generated no signal (A). In the gill tissue, CA transcripts mainly localized to the epithelial cell nuclei and hemolymph vessel under freshwater (B), low salinity (C), and high salinity (D) conditions. MC, marginal channel; (C) cuticle; N, epithelial cell nuclei; HV, hemolymph vessel. Scale bar: 50 μm.