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[Preprint]. 2022 Jun 7:rs.3.rs-1354127. [Version 2] doi: 10.21203/rs.3.rs-1354127/v2

Figure 2. Characteristics of the interactome and validation of novel SARS-CoV-2-human interactions.

Figure 2.

(a) UpSet plot showing the overlap of SARS-CoV-2-human protein-protein interactions from four studies (Table S3). Each bar shows the interactions shared by only the marked studies at the bottom. Composition of each bar in terms of the source of the interactions are indicated by different colors. (b) Co-immunoprecipitation confirming ORF3a-ZNF579 interaction in HEK 293T cells following transfection with ORF3a-FLAG or empty vector. (c) Western blot showing levels of ZNF579 along with GAPDH as a loading control in 293T cells following transfection with ORF3a-FLAG or empty vector. (d) ChIP-seq for ZNF579 in MCF7 cells from the ENCODE consortium at the HSPA6 locus. Signal is log2 fold change over input. (e) Expression of HSPA6 after transfection with ORF3a-FLAG or empty vector. Two transfection replicates were probed with two primer pairs to HSPA6 at three different template dilutions in technical triplicate (18 total reactions for each condition). Expression is normalized to GAPDH and then to the empty vector average using the double-delta Ct method. (f-g) Co-immunoprecipitation confirming ORF7b-STT3A and ORF7b-Sec61 interactions in HEK293T cells following transfection with ORF7b-FLAG or empty vector and STT3A-MYC or Sec61-V5, respectively. (h) Co-immunoprecipitation confirming N-histone H1.4 interaction in HEK293T cells following transfection with N or empty vector and histone H1.4.