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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: J Immunol. 2022 Apr 8;208(9):2085–2097. doi: 10.4049/jimmunol.2100926

Figure 5: TLR4-SNP mice accumulate more eosinophils after exposure to HDM extract by differential cell counting.

Figure 5:

WT and TLR4-SNP mice were treated with either HDM extract or endotoxin-free PBS according to the timeline (A). Groups of mice were treated with PBS or 50 μg of HDM on days 0, +5, +10, +15, with euthanasia on day +17. Lungs were lavage with 1 mL of PBS to obtain BAL and was used for cell counts by Cytospin followed by differential counting (B, C). The mean percentages of eosinophils, macrophages, neutrophils, and lymphocytes in each treatment group (B) are representative of 3 individual trials. The percentages of eosinophils and macrophages (C) were aggregated from 3 independent trials (n=4/treatment group/trial for PBS-treated groups, n=6/treatment group/trial for HDM-treated groups) with each point representing an individual mouse. Open circles designate females and closed circles designate males. All data was analyzed by 2-way ANOVA with Tukey’s Multiple Comparisons Test: *, P<0.05; **, P<0.005; ***, P<0.0005; ****, P<0.0001. Error bars represent mean ± SEM.