Fig. 3. Tumor LV drainage functions are impaired upon adoptive T cell transfer.

(A and B) C57BL/6 mice were injected or not with different doses of in vitro activated OT-1 cells (0.05 × 10⁶ or 0.5 × 10⁶ cells) 9 days after B16F10-OVA⁺VC⁺ inoculation and euthanized 5 days later. FITC-labeled beads (0.5 mm) were injected intratumorally 24 hours before euthanasia. Frequencies of (A) FITC beads⁺ DCs (CD11c⁺MHCII⁺) and (B) total DCs in TdLNs (pool of inguinal and axillary LN) by flow cytometry. Data represent two pooled experiments, N = 10 to 11 mice per group. (C) C57BL/6 mice were inoculated with B16F10-OVA⁺VC⁺ and were injected or not with 0.5 × 10⁶ in vitro activated OT-1 cells 9 days later. CTV-labeled B16F10-VC⁺ tumor cells were injected peritumorally at day 13, and mice were euthanized at day 14. Frequencies of GP38⁺ CTV⁺ cells in the TdLNs (inguinal and axillary). Data represent a pool of three experiments, N = 14 to 15 mice per group. (A and B) One-way ANOVA; (C) Mann-Whitney statistical test. Error bars show means ± SEM. *P < 0.05, **P < 0.01.