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. 1999 Dec;65(12):5619–5623. doi: 10.1128/aem.65.12.5619-5623.1999

FIG. 2.

FIG. 2

Shaking-flask experiments with mineral medium and citrate as the carbon source with recombinant strains synthesizing xylene oxygenase or styrene monooxygenase. Upper graphs show growth behavior, and lower graphs show the development of specific activity after induction with 0.05% (vol/vol) DCPK. The point of induction is indicated by the arrow. (A) Influence of inducer on the growth of parent strain P. putida KT2440. No styrene oxidation activity was observed for this strain irrespective of the induction. (B) Expression of chromosomally located (left graphs) and chromosomally and plasmid located (right graphs) xylene oxygenase genes. The structure of the gene cassette is indicated on top. (C) Expression of chromosomally located (left graphs) and chromosomally and plasmid located (right graphs) styrene monooxygenase genes. Closed symbols, uninduced cultures; open symbols, induced cultures.