Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 25;13:903978. doi: 10.3389/fphar.2022.903978

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Yakhnitsa, Ji, Hein, Presto, Griffin, Ponomareva, Navratilova, Porreca and Neugebauer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Location of CRF neurons, ChR2 injections for optogenetics, and microdialysis probes in the CeA. (A) Confocal image of mCherry labeled CRF neurons in a Crh-Cre rat 5 weeks post viral (AAV5- EF1α-DIO-mCherry, red) injection into CeA. Glutamatergic terminals expressing channelrhodopsin 2 (ChR2-eYFP) 5 weeks post viral injection (rAAV5/CaMKIIa-ChR2(H134R)-eYFP, green) into the external parabrachial nucleus (PB). CeM, CeL, CeC, medial, lateral, and capsular divisions of amygdala; BLA, basolateral amygdala. (B) Injection site for rAAV5-CaMKIIa-hChR2eYFP in the external lateral PB for ChR2 expression in glutamatergic neurons. SPC, superior cerebellar peduncle. (C) Diagrams show coronal brain slices at different anterior-posterior levels. Filled circles indicate the positions of the microdialysis probe tips for drug application into CeA that were included in this study.