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. 2022 Jun 8;8(23):eabn5345. doi: 10.1126/sciadv.abn5345

Fig. 1. Exogenous GLP-1 decreases blood glucose level in the DMH.

Fig. 1.

(A) Experimental paradigm for ICV injection GLP-1R agonist Exn4 experiment in WT mice. (B) ICV Exn4 caused decrease in fasting blood glucose. *P < 0.05 and ***P < 0.001 (t test). (C) To investigate DMH GLP-1R neuron response to Exn4 injection, we injected adeno-associated virus (AAV)–DIO–mCherry in the DMH of GLP-1R-Cre mice and stained with c-Fos antibody. The representative images showed that ICV Exn4 did increase c-Fos expression in GLP-1R neurons within the DMH. (D) Quantification of c-Fos–positive cell ration (relative to total GLP-1R neurons). ***P < 0.001 (t test). (E) Experimental paradigm for Exn4 injection in DMH of WT mice. (F) Fasting blood glucose decreased after Exn4 injection, an effect that was antagonized by the cAMP-PKA blocker, cAMP-Rp. *P < 0.05, **P < 0.01, and ***P < 0.001 [analysis of variance (ANOVA)], vehicle versus Exn4; #P < 0.05 (ANOVA), Exn4 versus Exn4 + cAMP-Rp (ANOVA). Numbers of animals are indicated in each panel.