Figure 5. Immune complexes engagement with CD32 induces proliferation in HIV-i-nfected CD32^dim cells.

Cells from healthy donors were infected with the viral strain HIV_BaL and 5 days post-infection Ki67 expression was measured by flow cytometry. (A) Expression of the proliferation marker Ki67 in uninfected or ex vivo HIV-infected CD4⁺ T cells. Median and ranges are shown (n=17). Statistical comparisons consisted of the Wilcoxon matched-pairs signed-rank test. (B) Percentage of Ki67⁺ cells in HIV-infected T_CD32^dim and T_CD32⁻ subsets are shown (n=17). Median and ranges are shown. Statistical comparisons consisted of the Wilcoxon matched-pairs signed-rank test. (C) Percentage of Ki67 expression on T_CD32^dim cells after immune complexes engagement (Plasma HIV⁺). Medium alone, FcRs blockers and plasma from an HIV-negative individual were included as controls. Median with interquartile range is shown (n=8). Statistical comparisons were performed using the Wilcoxon matched-pairs signed-rank test (comparison between p24⁺ and p24⁻ within each condition), and the ANOVA Friedman with Dunn’s multiple comparison test (comparison between different conditions of the p24⁺ or p24⁻ cells). (D) Percentage of Ki67 expression on HIV-infected T_CD32⁻ cells incubated under the same experimental conditions as shown in (C). Median with interquartile range is represented (n=8). Statistical comparisons consisted of the Wilcoxon matched-pairs signed-rank test.*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.