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. 2022 Jun 8;11:e72780. doi: 10.7554/eLife.72780

Figure 5. Ribosome without RPL3 methylation shows higher processivity on Tyr codons in vitro.

(A) Schematic representation of the hybrid translation system and the processivity reporter. (B) The box plot for the relative ratio of SlopeFluc to SlopRluc for the reporter with Tyr repeat insertion. Data from seven replicates (points) are shown. Significance was determined by Brunner–Munzel test (unpaired, two-sided).

Figure 5—source data 1. Primary data for graphs in Figure 5B.

Figure 5.

Figure 5—figure supplement 1. Characterization of hybrid translation system and Renilla-firefly fused reporter.

Figure 5—figure supplement 1.

(A) In vitro translation from firefly luciferase was conducted in the indicated materials. Data from three replicates (points) and the mean (bar) with SD (error bar) are shown. RRL, rabbit reticulocyte lysate. (B) The box plot for the relative ratio of SlopeFluc to SlopRluc for the reporters with and without Tyr repeat insertion. Data from three replicates for no insertion reporter and seven replicates for Tyr repeat-inserted reporter (points) are shown. Significance was determined by the Student’s t-test (unpaired, two-sided).
Figure 5—figure supplement 1—source data 1. Primary data for graphs in Figure 5—figure supplement 1A, B.