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. 2022 Jun 8;11:e72780. doi: 10.7554/eLife.72780

Figure 6. METTL18 deletion leads to cellular proteotoxicity.

Figure 6.

(A) Microscopic images of FlucWT-EGFP or FlucDM-EGFP in naïve and METTL18 knockout (KO) HEK293T cells. Arrowhead, protein aggregation; scale bar, 10 μm. (B) Quantification of cells with Fluc-EGFP aggregates. Data from three replicates (points) and the mean (bar) with SD (error bar) are shown. Significance was determined by Student’s t-test (unpaired, two-sided). (C) Western blot for FlucDM-EGFP (probed by anti-GFP antibody) expressed in naïve and METTL18 KO HEK293T cells treated with MG132 (0.25 μM for 24 hr). β-Actin was probed as a loading control.

Figure 6—source data 1. Full and unedited images corresponding to Figure 6A.
Figure 6—source data 2. Full and unedited blots corresponding to Figure 6C.
Figure 6—source data 3. Primary data for graphs in Figure 6B.