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. 2022 Jun 8;11:e72780. doi: 10.7554/eLife.72780

Figure 8. METTL18 deletion degrades Tyr-rich proteins by proteasome.

Figure 8.

(A) Schematic representation of SILAC-MS for total proteins. (B, C) Cellular protein abundance changes in METTL18 knockout (KO) cells with the treatment of proteasome inhibitor MG132 and the control DMSO, assessed by SILAC-MS (n = 2). The relative abundance in METTL18 KO HEK293T cells compared to naïve HEK293T cells is calculated. Data with DMSO or MG132 treatment (B) and fold enrichment (MG132 compared to DMSO) (C) are shown ranked by the fold enrichment in (C). Proteins with 1.5 or higher enrichment by MG132 treatment are highlighted. Tyr-rich proteins are defined as proteins with 30 or more Tyr residues.