FIGURE 2.

Reciprocal co-immunoprecipitation was performed on lysate extracted from rat kidney cortex using magnetic beads coupled to anti-ANT2 and anti-UCP2 antibodies. (A), ANT2 co-purified with UCP2 when using beads coupled to anti-UCP2 antibodies. ANT and UCP inhibitors interfered with the ANT2-UCP2 interaction. (B), Similarly, UCP2 co-purified with ANT2 when using beads coupled to anti-ANT2 antibodies. The lane representing beads control revealed binding between the magnetic beads and UCP2, although to a smaller extent than in the lane representing the ANT2. The same inhibitor specific interaction interference was observed, as the band densities did not exceed the apparent unspecific binding between the beads and UCP2.