Table 2.

Differences in Ct values and respective p values, for factors dilution and heating time, for genes ORF1ab, N, and E, as determined by post hoc Tukey HSD analysis.

Factors		Gene ORF1ab		Gene N		Gene E
		Ct difference	p value	Ct difference	p value	Ct difference	p value
Dilution	1 : 10–1 : 1	0.390	0.4569	0.558	0.5582	0.360	0.2144
	1 : 100–1 : 10	−1.013	0.0206	−1.138	0.0270	−0.566	0.0399
Heating time	10–5	−3.645	3.7 × 10−6	−4.030	7.2 × 10−6	−3.095	2.0 × 10−7
	15–10	−1.957	0.0011	−2.240	0.0014	−1.830	3.61 × 10−5
	20–15	0.487	0.5560	0.423	0.7609	0.263	0.6736