Figure 3.
Effect of AR and GL extracts on Dox-induced expression of proapoptotic proteins in H9c2 cells. H9c2 cells were pretreated with 1 mg/mL of AR or GL extract or vehicle (control) for 12 h and then treated with or without Dox (0.5 μM) for 24 h. Protein expression levels of PARP, cleaved-PARP, caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, Bax, Bcl-2, and β-actin were examined using western blot analysis. (a) Representative blots. (b–d) Quantitative analysis of the ratios of protein expression level of (b) cleaved PARP/PARP, (c) cleaved caspase-3/caspase-3, (d) cleaved caspase-9/caspase-9, and (e) Bax/Bcl-2. Data are presented as percentage of control group values (mean ± SD of three independent experiments). ∗p < 0.05 indicates a statistically significant difference.