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. 2022 Jun 1;606(7913):343–350. doi: 10.1038/s41586-022-04786-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Experimental approach. b, Sorting of single human HSC/MPP and HPCs from cord blood, peripheral blood and bone marrow. Cells were stained with the panel of antibodies in Supplementary Table 1 then single HSC/MPP or HPCs were index sorted according to the strategy depicted into individual wells of 96 well plates. Image created with FlowJo v10. c, Colony forming efficiency per individual of all single HSPCs sorted. d, Box-and-whisker plots showing fluorescence intensity for different cell surface markers used to define human HSCs, with different patients in rows. CD90 and CD49f are markers used to define the short and long term HSC subsets, which were included in our panel but were not used in sorting. Cells that produced colonies large enough to sequence are shown in teal; cells that did not form large enough colonies to sequence are in orange. The horizontal lines denote the median, the boxes the interquartile range and the whiskers the range. The number (n) of ‘sequenced’ (teal) and ‘not sequenced’ (orange) colonies included for each individual are shown to the right of each panel.