TABLE 1.
Summary of DNA concentration and quality metrics. Technical replicates are denoted as “XX_1” and “XX_2”
| Extraction methodology | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PB | PE | UC | GT | PC | AE | ||||||||
| Method | Metric | PB_1 | PB_2 | PE_1 | PE_2 | UC_1 | UC_2 | GT_1 | GT_2 | PC_1 | PC_2 | AE_1 | AE_2 |
| Qubit | DNA concentration (µg ml–1) | 2.34 | 1.71 | 4.08 | 4.21 | 30.7 | 36.2 | 110 | 110 | 75.8 | 77.9 | 90.6 | 95.5 |
| Nanodrop | A260/A280 a | 6.01 | 5.53 | 1.75 | 2.27 | 1.95 | 1.74 | 1.74 | 1.82 | 1.97 | 1.95 | 1.8 | 1.76 |
| Nanodrop | A260/A230 b | 0.26 | 0.16 | 0.33 | 0.08 | 1.98 | 2.02 | 1.86 | 2.27 | 2.07 | 2.07 | 1.56 | 1.36 |
a
Primary measure of nucleic acid purity—expected value for “pure” DNA: ~1.8 while >2 indicates RNA contamination.
b
Secondary measure of nucleic acid purity—evaluates residual chemical contamination (phenol, guanidine HCl, carbohydrate carryover). Expected values are in the range of 1.8–2.2. Values significantly lower indicate chemical contamination and are undesired.