TABLE 4.

Comparison of sequencing run read metrics between untreated and BluePippin size‐selected samples. All percentages are relative to the total reads (or sequencing yield) of the quality filtered reads

	Untreated		BluePippin high‐pass size selection
	GT_1	GT_2	GT_1 SS	GT_2 SS
All reads
Total reads	2,052,300	3,681,083	221,344	430,986
Sequencing yield (Gbp)	1.440	1.959	0.410	0.450
Human reads
Number of reads	1,584,229	2,674,013	117,071	282,113
%	77.19	72.64	52.89	65.46
Sequencing yield (Gbp)	0.908	1.199	0.104	0.162
%	63.07	61.23	25.31	35.92
Microbial reads
Number of reads	468,071	1,007,070	104,273	148,873
%	22.81	27.36	47.11	34.54
Sequencing yield (Gbp)	0.532	0.759	0.306	0.289
%	36.93	38.77	74.69	64.08
N50	2810	1929	6106	5594
Microbial reads >2.5 kb
Number of reads	43,173	49,572	40,248	34,949
%	2.10	1.35	18.18	8.11
Sequencing yield (Gbp)	0.278	0.352	0.252	0.215
%	19.32	17.98	61.26	47.74
Microbial reads >20 kb
Number of reads	1269	2294	300	407
%	0.06	0.06	0.14	0.09
Sequencing yield (Gbp)	0.035	0.067	0.008	0.011
%	2.42	3.41	1.86	2.49

GT, Qiagen Genomic Tip 20/G with enzymatic treatment; SS size‐selection.