TABLE 6.
flye assembly statistics. Assemblies were polished using pilon and short‐reads from the extraction PB_2
| Qiagen Genomic Tip (GT) | Phenol–chloroform (PC) | Agarose encasement (AE) | ||||
|---|---|---|---|---|---|---|
| GT_1 | GT_2 | PC_1 | PC_2 | AE_1 | AE_2 | |
| Total length (bp) | 28,002,213 | 35,034,984 | 10,630,822 | 11,909,082 | 17,557,138 | 39,151,308 |
| Number of contigs | 401 | 466 | 137 | 106 | 483 | 855 |
| No.of contigs >5 kb | 369 | 412 | 130 | 101 | 467 | 775 |
| No. of contigs >10 kb | 347 | 383 | 124 | 100 | 440 | 714 |
| No. of contigs >20 kb | 299 | 336 | 107 | 97 | 354 | 564 |
| No. of contigs >50 kb | 159 | 194 | 73 | 67 | 84 | 203 |
| No. of contigs >100 kb | 63 | 84 | 33 | 35 | 21 | 69 |
| Longest contig (bp) | 1,025,627 | 1,176,789 | 504,505 | 676,998 | 304,763 | 875,613 |
| Shortest contig (bp) | 512 | 528 | 2103 | 1025 | 913 | 511 |
| N50 (bp) | 129,677 | 155,366 | 122,828 | 166,496 | 44,461 | 71,755 |
| Number of genes | 32,540 | 41,818 | 11,628 | 13,025 | 21,392 | 48,784 |
| Single‐copy core genes | ||||||
| Bacteria_71 | 845 | 1072 | 298 | 339 | 551 | 1163 |
| Archaea_76 | 428 | 554 | 139 | 167 | 283 | 614 |
| Protista_83 | 34 | 48 | 11 | 14 | 26 | 54 |
| Ribosomal RNAs | 75 | 116 | 30 | 43 | 44 | 100 |
| No. of expected bacterial genome | 12 | 12 | 5 | 5 | 7 | 15 |
| Circular contigs | ||||||
| Number of circular contigs | 20 | 32 | 8 | 5 | 9 | 24 |
| Max. length | 86,329 | 155,366 | 155,422 | 155,411 | 24,494 | 88,098 |