Skip to main content
. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: SLAS Discov. 2022 Jan 31;27(4):209–218. doi: 10.1016/j.slasd.2022.01.003

Figure 1.

Figure 1.

A multiparametric calcium fluorescence assay for assessing neural cell activity in cortical spheroids. (a.) Schematic showing the timeline of the experiment. StemoniX cortical spheroids are maintained for 1-week prior to imaging with the FLIPR. Calcium 6 (Cal6) dye is added and 2-hrs later, baseline activity is recorded; 5-min after the baseline recording, compounds are added to spheroids via acoustic dispensing, and calcium activity is recorded again 90-min after compound treatment. FLIPR recordings are 8-min. (b.) Schematic defining peak parameters obtained from Peak Pro 1.0 analysis of calcium imaging data. PkCt = Peak Count; PkS = Peak Spacing; PkW = Peak Width; PkA = Peak Amplitude for whole peak; PkA10 = Peak Amplitude beginning at 10% peak height; PkRt = Peak Rise Time; PkDt = Peak Decay Time. (c.) Representative time series plots showing calcium activity after treatment with control compounds that should reliably show activity changes based on cell types represented. Plots are displayed as percent change in calcium fluorescence over baseline, which is considered the first 2 seconds of the recording. (d.) Scatter plots showing the percent change from the DMSO average for all compounds tested across each peak parameter used in the HCP analysis. Compounds from 1c are highlighted, and data is shown in comparison to DMSO-treated wells in iPSC-derived cortical spheroids are highlighted, along with stimulatory (4-AP) and inhibitory (muscimol) controls.