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Results of purification of NanB sialidase by anion exchange chromatography.
| Anion exchange chromatography fraction | Supernatant volume (ml) | Total protein (mg) | Total sialidase activity (U) | Specific activity (U/mg) |
|---|---|---|---|---|
| Ccrude protein | 100 | 64.80 | 16.80 | 0.259 |
| F0 | 100 | 8.60 | 15.40 | 1.790 |
| F1 (0.2 M NaCl) | 5 | 10.66 | 0.14 | 0.013 |
| F2 (0.4 M NaCl) | 5 | 11.13 | 0.02 | 0.002 |
| F3 (0.6 M NaCl) | 5 | 1.82 | 0.01 | 0.006 |
| F4 (0.8 M NaCl) | 5 | 0.02 | 0.001 | 0.023 |
| F5 (1 M NaCl) | 5 | 0.03 | 0.001 | 0.020 |
