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. Author manuscript; available in PMC: 2022 Nov 2.
Published in final edited form as: Nat Metab. 2022 May 2;4(5):627–643. doi: 10.1038/s42255-022-00566-0

Extended Data Fig. 4. TrkB.T1 in VMH astrocytes is an essential regulator of body weight under standard chow conditions.

Extended Data Fig. 4

a. Expression of the neuronal marker b3 tubulin (FT n = 5, Astrocyte n = 7) and

b. GLT-1 in mRNA isolated from VMH astrocytes and flowthrough (FT n = 3, Astrocyte n = 3).

c. Immunolabeling of TrkB.T1 in the VMH of a TrkB F/F animal injected unilaterally with AAV5-GFAP-GFP-Cre 4 weeks post-surgery. Scale bar 15 uM.

d. Co-localization of AAV5-GFAP-GFP-Cre with GFP signal with astrocytes (Sox9 + cells) and exclusion from neurons (NeuN + cells). Scale bar 50 uM.

e. Co-localization of AAV5-GFAP-driven GFP signal with astrocytic (Sox 9), neuronal (NeuN) and microglial (Iba)-specific markers. Scale bar 150 nM.

f. Image showing that viral spread is limited to the VMH.

g. Western blot and analysis showing TrkB.T1 expression in Control and TrkB.T1 KD mice within the VMH (n = 6) and the DMH of mice (n = 5). Data collected from one experiment. Student’s two-sided t-test, * , p = 0.01. Data represented as mean +/− SEM.